|The p53 tp53 (Catalog #MBS212262) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI p53 (aa20-25) reacts with Bovine, Cat, Green Monkey, Horse and may cross-react with other species as described in the data sheet. MyBioSource\'s p53 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, ELISA (EIA), Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB).
Immunohistology - Paraffin: Maximum Dilution: 1/25; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Maximum Dilution: 1/1000. Researchers should empirically determine the suitability of the p53 tp53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The p53 tp53 product has the following accession number(s) (GI #120407068) (NCBI Accession #NP_000537.3) (Uniprot Accession #P04637). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI p53 (aa20-25) with the following immunoassay(s):
Testing Data (Western blot analysis of COS-7 simian fibroblast-like whole cell lysate probed with Mouse anti p53 antibody followed by HRP conjugated Goat anti Mouse IgG, visualized using chemiluminescence)
Testing Data (Published customer image:Response of p53siRNA U2-OS cells to etoposide. (A) Inhibition of p53 expression in p53siRNA U2-OS. Actin was used as loading control. (B) p53siRNA U2-OS were less sensitive to etoposide when compared with parental and Ctrl U2-OS;). Student\'s test from three independent experiments indicated significantly higher IC50 mean values at 72 h of treatment in p53siRNA U2-OS than in Ctrl and parental U2-OS cells; p = 0.05 (C) Etoposide treatment did not induce mature miR-34a expression in p53siRNA U2-OS, as opposed to Ctrl U2-OS. (D) p53siRNA U2-OS cells presented CpG island methylation (M-MSP) of one of the two alleles of miR-34a. In Ctrl U2-OS both alleles were unmethylated. (E) p53siRNA transfection determined lengthening of G2/M phase after 48 h of etoposide treatment when compared to untreated cells. (F) Western blot of cyclin D1 and CDK4 in p53siRNA cell showed increased amount of CDK4 linked to cyclin D1 and total CDK4 after etoposide treatment when compared to control. No differences in cyclin D1 levels were seen. Ctrl = siRNA negative control duplex; C = Untreated cells; T = Etoposide treated cells.From: Novello C, Pazzaglia L, Conti A, Quattrini I, Pollino S, et al. (2014) p53-Dependent Activation of microRNA-34a in Response to Etoposide-Induced DNA Damage in Osteosarcoma Cell Lines Not Impaired by Dominant Negative p53 Expression. PLoS ONE 9(12): e114757.)
Testing Data (Published customer image: p53 protein expression in OS cells. wt-p53 U2-OS, U2-OS transfected with empty vector (U2-OS/e) and p53-impaired U2-OS175 cells were positive to anti-p53 that binds the transactivation site of N-terminal domain (aa20-25), with increased expression in U2-OS175 cells. U2-OS and U2-OS/e also presented accumulation of p53 phosphorylated at Ser20 residue (p-p53). MG63 and Saos-2 were negative to both antibodies. Actina was used as loading control.From: Novello C, Pazzaglia L, Conti A, Quattrini I, Pollino S, et al. (2014) p53-Dependent Activation of microRNA-34a in Response to Etoposide-Induced DNA Damage in Osteosarcoma Cell Lines Not Impaired by Dominant Negative p53 Expression. PLoS ONE 9(12): e114757.)
Mouse anti Human p53 antibody, clone DO-1 recognizes the human p53 tumor suppressor protein, also known as cellular tumor antigen p53 or NY-CO-13. Clone DO-1 binds to both wild type and mutant forms of the p53 protein found in various malignancies (Kern et al. 1992). p53 is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells (Haupt et al. 2003) and thus functions as a tumor suppressor that is involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancer (Joerger andFersht 2007) Mouse anti Human p53 antibody, clone DO-1 recognizes an epitope at the N-terminal end of p53 between amino acids 20-25,common to isoforms 1-3 of p53.