|The p27Kip1 cdkn1b (Catalog #MBS301470) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-Human p27Kip1 Polyclonal Antibody reacts with Human
Predicted Reactivity: Dog, Hamster, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s p27Kip1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin.
Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Immunohistochemistry Dilution: 1:100
IHC Positive Control: Prostate, Colon. Researchers should empirically determine the suitability of the p27Kip1 cdkn1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The p27Kip1 cdkn1b product has the following accession number(s) (GI #4757962) (NCBI Accession #NP_004055.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit Anti-Human p27Kip1 Polyclonal Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Human prostate stained with anti-p27Kip1 antibody)
Recognizes a protein of 27kDa, identified as p27Kip1. p27Kip1 is a candidate tumor suppressor gene and functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-b induced G1 arrest.
Immunogen: Synthetic peptide derived from C-terminus of human p27Kip1.
Control Tissue: Colon, Prostate
Cellular Localization: Nucleus
Entrez Gene Code: 1027
Pathway: Cell Cycle. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing p27Kip1 are readily searchable from our website. Different antibodies against the same target such as p27Kip1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.