anti-p18INK4c antibody product blog
Tags: Antibody; Monoclonal Antibody; p18INK4c; anti-p18INK4c antibody;
The p18INK4c n/a (Catalog #MBS600241) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The p18INK4c (BSA & Azide Free) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s p18INK4c can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF).Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry.
Dilution: Immunoprecipitation: 1-2ug/mg. Co-precipitates cdk4. Use Protein G. For optimum results, immunoprecipitate with Western blot.
Western Blot: 1-2ug/ml for 2hrs at RT.
Immunohistochemistry (formalin/paraffin): 1:50 for 30 min at RT. Staining of formalin/paraffin tissues is required. Boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Researchers should empirically determine the suitability of the p18INK4c n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the p18INK4c (BSA & Azide Free) with the following immunoassay(s):
Western Blot (WB) (Western Blot using MBS600241)
Western Blot (WB) (Western Blot using MBS600241)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human tonsil stained with MBS600241 using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells.)
p18INK4c is a specific inhibitor of cdk4/cdk6 and is expressed during G1-to S transition in the eukaryotic cell division cycle. It is maximally induced as cells enter S-phase.
Immunogen: Purified recombinant p18INK4c protein. Cellular Localization: Nuclear and cytoplasmic. Positive Control: HeLa cells, Tonsil, skeletal muscle, heart and pancreas. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing p18INK4c are readily searchable from our website. Different antibodies against the same target such as p18INK4c may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.