anti-OX-2 antibody product blog
Tags: Antibody; Monoclonal Antibody; OX-2; anti-OX-2 antibody;
The OX-2 n/a (Catalog #MBS520397) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rat OX-2 PE (Clone OX-2) (mouse IgG1) reacts with Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s OX-2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the OX-2 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Rat OX-2 PE (Clone OX-2) (mouse IgG1) with the following immunoassay(s):
Testing Data
mouse anti-rat OX-2 monoclonal antibody recognizes a monomorphic determinant present on rat thymocytes, brain, follicular dendritic cells in lymphoid organs, vascular endothelium, and at low levels on some smooth muscle and B lymphocytes. The purified brain and thymocyte OX-2 antigens are glycoproteins with apparent M.W. of 41 KDa and 47 KDa respectively. The amino acid composition of brain and thymocyte OX-2 antigen are very similar and are antigenically similar to those found on other tissues. The carbohydrate composition shows that this antigen is highly glycosylated (brain OX-2 - 24% and thymocyte OX-2 - 33% carbohydrate by weight). The OX-2 antigen shows similarities to the Thy-1 antigen in its odd pattern of tissue distribution, carbohydrate composition and characteristic migration on SDSPAGE. Also, the OX-2 antigens, like Thy-1 antigens, have homologies with immunoglobulin domains; the overall structure of OX-2 is similar to an Ig light chain or the T cell receptor b chain. Because of its distribution, it is thought to play a role in mediating recognition events at cell surfaces. This clone can be used in flow cytometry, affinity chromatography, immunohistochemistry and in binding assays. It is also useful for labeling the follicular dendritic cells thought to be involved in the generation of B cell memory as it does not label the Ia-positive dendritic cells present in the T-dependant areas of lymphoid organs.
Hybridoma Production: Spleen cells from BALB/c mice immunized with rat thymocyte membrane glycoproteins were fused with mouse NS-1 myeloma cells. Presentation: 50 ug or 300 ug R-PE conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing OX-2 are readily searchable from our website. Different antibodies against the same target such as OX-2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.