anti-Oncofetal Antigen 5T4 antibody product blog
Tags: Antibody; Monoclonal Antibody; Oncofetal Antigen 5T4; anti-Oncofetal Antigen 5T4 antibody;
The Oncofetal Antigen 5T4 n/a (Catalog #MBS606018) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Oncofetal Antigen 5T4 (TPBG, Trophoblast Glycoprotein, 5T4 Oncofetal antigen, 5T4 Oncofetal Trophoblast Glycoprotein, M6P1) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Oncofetal Antigen 5T4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Flow Cytometry (FC/FACS).Suitable for use in Direct ELISA and Flow Cytometry.
Dilution: Flow Cytometry: 2.5ug/10e6 cells. Tested with BG01V human embryonic stem cells. Researchers should empirically determine the suitability of the Oncofetal Antigen 5T4 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Oncofetal Antigen 5T4 (TPBG, Trophoblast Glycoprotein, 5T4 Oncofetal antigen, 5T4 Oncofetal Trophoblast Glycoprotein, M6P1) with the following immunoassay(s):
Testing Data (Lymphocytes gated PBMCs stained with 367100 (clone OKT1, red histogram). PBMCs lymphocytes stained with IgG1 APC isotype control (blue histogram).)
Oncofetal antigen 5T4, also TPBG and trophoblast glycoprotein, is a 72kD glycoprotein member of the LRR family of proteins. It is expressed on trophoblasts, tumor cells, ovarian cuboidal epithelium and embryonic stem cells, and impacts cell adhesion and motility. The human 5T4 cDNA encodes a type I transmembrane protein precursor that is 420 amino acids in length. It contains a 324aa extracellular region (aa 32-355) that shows one Ser-rich region followed by seven Leu-rich repeats (aa90-355). Over aa31-355, human 5T4 shares 81% and 85% aa identity with mouse and canine 5T4, respectively.
Immunogen: Recombinant protein corresponding to aa31-355 of human Oncofetal Antigen 5T4 expressed in NSO cells. (Q13641). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Oncofetal Antigen 5T4 are readily searchable from our website. Different antibodies against the same target such as Oncofetal Antigen 5T4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.