anti-OCT4 antibody product blog
Tags: Antibody; Polyclonal Antibody; OCT4; anti-OCT4 antibody;
The OCT4 n/a (Catalog #MBS617538) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Oct4 (POU Domain, Class 5, Transcription Factor 1 Oct3/4, POU5F1, HGNC:9221, MGC22487, Octamer-binding Transcription Factor 3, Oct3, OTF3, OTF4, POU-type Homeodomain-containing DNA-binding Protein) reacts with Human, Rodent and may cross-react with other species as described in the data sheet. MyBioSource\'s OCT4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA).Suitable for use in ELISA.
Dilution: ELISA (Serum Titer): 1:200,000. Researchers should empirically determine the suitability of the OCT4 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The OCT4 n/a product has the following accession number(s) (GI #333973470) (NCBI Accession #AEG42188.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Oct-4 (POU5F1) is a transcription factor that is expressed in undifferentiated embryonic stem cells and germ cells during normal development (1). A network of key factors that includes Oct-4, Nanog and Sox2, is necessary for the maintenance of pluripotent potential. Downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Oct-4 has also been shown to be an useful marker for germ cell tumors (4). Stem cells are crucial ingredients in the progressive restriction of cellular developmental capacity during mammalian embryogenesis. Until recently, only embryonic stem cells (ESC) had been shown to exhibit the potential to differentiate into cells of the all three germ layers. However, adult stem cells (ASC) can regain pluripotency (potential to differentiate into various lineages) if transferred from one stem-cell microenvironment into another. The transcription factor OCT4, expressed in ESCs and germ cells, is strongly implicated in the process of maintaining as well as regaining stem-cell pluripotency and functions as a key regulator of mammalian germline development. Oct3/4 maintains developmental potency and governs distinct fates of embryonic stem cells. A moderate increase in expression causes differentiation into primitive endoderm and mesoderm. In contrast, repression of Oct3/4 induces loss of pluripotency and dedifferentiation to trophectoderm. Thus, a critical amount of Oct3/4 is required to sustain stem cell self-renewal, and up- or downregulation induces divergent developmental programs. Data suggest that Oct3/4 is a master regulator of pluripotency that controls lineage commitment.
Immunogen: Synthetic peptide (16aa) aa282-297: RQKGKRSSSDYAQRED, coresponding to human Oct-4 (KLH). MW: of antigen: 38,.7kD. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing OCT4 are readily searchable from our website. Different antibodies against the same target such as OCT4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.