anti-NR4A1 antibody product blog
Tags: Antibody; anti-NR4A1 antibody; NR4A1; Polyclonal Antibody; NUR77;
The NR4A1 nr4a1 (Catalog #MBS175646) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NUR77 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NUR77 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the NR4A1 nr4a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The NR4A1 nr4a1 product has the following accession number(s) (GI #127819) (NCBI Accession #P22736.1) (Uniprot Accession #P22736). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-NUR77 antibody with the following immunoassay(s):
Western Blot (WB) (Anti-NUR77 antibody, MBS175646, Western blotting
Lane 1: A431 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: JURKAT Cell Lysate
)
Description: Rabbit IgG polyclonal antibody for Nuclear receptor subfamily 4 group A member 1(NR4A1) detection. Tested with WB in Human, Mouse, Rat.
Background: NR4A1(NUCLEAR RECEPTOR SUBFAMILY 4, GROUP A, MEMBER 1), also called NAK1, GFRP1, TR3, NUR77 or NGFIB, is a protein that in humans is encoded by the NR4A1 gene, which is also a member of the Nur nuclear receptor family of intracellular transcription factors. The NR4A1 gene is mapped on 12q13.13. NR4A1 is involved in cell cycle mediation, inflammation and apoptosis. It plays a key role in mediating inflammatory responses in macrophages. In addition, subcellular localization of the NR4A1 protein appears to play a key role in the survival and death of cells. Nr4a1 was overexpressed in Wnt1 -transformed mouse mammary cells. Nr4a1 was also induced by lithium, a Wnt1 mimic, and the Nr4a1 promoter was activated by lithium and beta-catenin, a Wnt1 downstream effector. In contrast, human NR4A1 was not upregulated by beta-catenin, indicating that this gene is regulated differently in human and mouse cells. Adenoviral expression of Nr4a1 induced genes involved in gluconeogenesis, stimulated glucose production both in vitro and in vivo, and raised blood glucose levels.
Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human NUR77 (99-112aa ASFKFEDFQVYGCY), identical to the related rat and mouse sequences. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NR4A1 are readily searchable from our website. Different antibodies against the same target such as NR4A1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.