anti-NR1H2 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-NR1H2 antibody; NR1H2; LXR beta/NR1H2;
The NR1H2 nr1h2 (Catalog #MBS421375) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-LXR beta/NR1H2 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. MyBioSource\'s LXR beta/NR1H2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC).Peptide ELISA: Antibody detection limit dilution 1: 64000.
Immunohistochemistry: In paraffin embedded Human Spleen shows nucleolar staining. Recommended concentration, 1-2ug/ml.
Western Blot: Experiments gave a band at approx 80kDa and a weak band at appr. 60-kDa in human bone marrow lysate after 1ug/ml antibody staining. These bands correspond to earlier findings with different antibodies (PMID: 12393874-60kDa and other unpublished antibodies-80kDa). This protein has a calculated MW of 51. 0kDa according to NP_009052. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the NR1H2 nr1h2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NR1H2 nr1h2 product has the following accession number(s) (GI #85362735) (NCBI Accession #NP_009052.3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
SSPTTSSLDT PLPGC.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-LXR beta/NR1H2 Antibody with the following immunoassay(s):
Western Blot (WB) ((1ug/ml) staining of human bone marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC) ((3ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunogen: Peptide with sequence SSPTTSSLDTPLPGC, from the N Terminus of the protein sequence according to NP_009052.3.
Epitope: N Terminus. Note: Based on the peptide used, this antibody is expected to be specific for LXR beta and not to cross-react with LXR alpha. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NR1H2 are readily searchable from our website. Different antibodies against the same target such as NR1H2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.