anti-NOTUM antibody product blog
Tags: Antibody; Polyclonal Antibody; NOTUM; anti-NOTUM antibody;
The NOTUM notum (Catalog #MBS151515) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NOTUM Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NOTUM can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).NOTUM antibody can be used for detection of NOTUM by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the NOTUM notum for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NOTUM notum product has the following accession number(s) (GI #194394139) (NCBI Accession #NP_848588) (Uniprot Accession #Q6P988). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NOTUM Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of NOTUM in human kidney tissue lysate with NOTUM antibody at (A) 0.5 and (B) 1 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of NOTUM in human brain tissue with NOTUM antibody at 2.5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of NOTUM in Mouse Kidney cells with NOTUM antibody at 20 μg/mL.)
NOTUM Antibody: In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by beta-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway.
Conjugate: Unconjugated
Immunogen: NOTUM antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human NOTUM.
Buffer: NOTUM Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NOTUM are readily searchable from our website. Different antibodies against the same target such as NOTUM may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.