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anti-NOTCH3 antibody product blog

Posted on 2014-01-31 08:56:25 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; NOTCH3; anti-NOTCH3 antibody;
The NOTCH3 notch3 (Catalog #MBS420398) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-NOTCH3 Antibody reacts with Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NOTCH3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC).
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Hippocampus shows select nuclear staining and weaker staining in different comparments of the neuronal cell bodies. Recommended concentration, 4-6ug/ml.
Western Blot: Preliminary experiments gave approx 60+70kDa bands in human brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 244kDa according to NP_000426.1. The 60+70kDa bands were successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the NOTCH3 notch3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The NOTCH3 notch3 product has the following accession number(s) (GI #134244285) (NCBI Accession #NP_000426.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-NOTCH3 Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) ((4ug/ml) staining of paraffin embedded Human Hippocampus. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC) NOTCH3.

Immunogen: Peptide with sequence C-QLGPQPEVTPKRQ, from the C Terminus of the protein sequence according to NP_000426.2.
Epitope: C Terminus. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NOTCH3 are readily searchable from our website. Different antibodies against the same target such as NOTCH3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Breast Neoplasms, CADASIL, Cardiovascular Diseases, Cell Transformation, Neoplastic, Cognition Disorders, Disease Models, Animal, Fibrosis, Inflammation, Lung Neoplasms, Neoplasm Metastasis are some of the diseases may be linked to Goat anti-NOTCH3 Antibody. Bone, Brain, Embryonic Tissue, Ganglia, Heart, Liver, Lung, Nerve, Skin, Vascular tissues are correlated with this protein.
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