anti-NOS1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-NOS1 antibody; NOS1; NOS1 / nNOS;
The NOS1 nos1 (Catalog #MBS241618) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal (IgG) to Human NOS1 / nNOS reacts with Human, Monkey, Mouse, Bat, Dog, Horse, Rabbit (tested or 100% immunogen sequence identity)Predicted Reactivity: Rat, Chicken (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. MyBioSource\'s NOS1 / nNOS can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP).
ICC, IHC-P (5 ug/ml), IP, WB
Usage: Immunohistochemistry: MBS241618 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS241618 was determined to be 5 ug/ml. Researchers should empirically determine the suitability of the NOS1 nos1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NOS1 nos1 product has the following accession number(s) (GI #10835173) (NCBI Accession #NP_000611.1) (Uniprot Accession #P29475). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit Polyclonal (IgG) to Human NOS1 / nNOS with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-nNOS / NOS1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC) (Anti-nNOS / NOS1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Western Blot (WB) (Western blot of NNOS / NOS antibody.)
NOS1 / nNOS belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5\' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.