|The NOP1 nop1 (Catalog #MBS500003) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Fibrillarin (Nop1p) reacts with Human, rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Fibrillarin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF).
Quality Control: Western blots performed on each lot.
IF: 1:500. Researchers should empirically determine the suitability of the NOP1 nop1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NOP1 nop1 product has the following accession number(s) (GI #6320190) (NCBI Accession #NP_010270.1) (Uniprot Accession #P15646). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Fibrillarin (Nop1p) with the following immunoassay(s):
Mouse monoclonal antibody.
Antigen: Yeast nuclear preparations.
Immunogen Information: Yeast nuclear preparations
Immunogen Species: Yeast
Packing: 100ul antibody per vial+10 mM NaN3. Adequate amount of material to conduct 10-mini Western Blots. Species Reactivity Note: The antibody has been directly tested for reactivity in human, rat, plant, Drosophila, C. elegans and S. pombe. It is expected that it will work on other mammal tissues.
Biological Significance: Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3). This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NOP1 are readily searchable from our website. Different antibodies against the same target such as NOP1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.