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anti-NLRC4 antibody product blog

Posted on 2015-07-11 18:30:57 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; Ipaf; anti-NLRC4 antibody; NLRC4;
The NLRC4 nlrc4 (Catalog #MBS151519) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ipaf Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Ipaf can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF).
Ipaf polyclonal antibody can be used for the detection of Ipaf by Western blot at 1 and 2 mug/mL. A 110 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 10 mug/mL. Researchers should empirically determine the suitability of the NLRC4 nlrc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The NLRC4 nlrc4 product has the following accession number(s) (GI #40788015) (NCBI Accession #NP_067032) (Uniprot Accession #Q9NPP4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Ipaf Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Ipaf in human PBL lysate with Ipaf antibody at 0.5 (lane A), 1 (lane B), and 2 (lane C) μg/mL, respectively.)
Western Blot (WB) NLRC4.

Immunocytochemistry (ICC) (Immunocytochemistry of Ipaf in THP-1 cells with Ipaf antibody at 10 μg/mL.)
Immunocytochemistry (ICC) NLRC4.

Immunofluorescence (IF) (Immunofluorescence of Ipaf in THP1 cells with Ipaf antibody at 10 μg/mL.)
Immunofluorescence (IF) NLRC4.

Ipaf Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adaptor molecules and proteases including several members of the caspase family. Another family of proteins that functions as a critical regulator of apoptosis and NFκ signaling pathways is the CED-4/Apaf-1 (apoptosis protein activating factor-1) protein family. Ipaf (ICE protease activating factor) is a CED-4/Apaf-1 family member that activates caspase-1/ICE and can induce apoptosis in human cells in a caspase-1 dependent manner. Ipaf and caspase-1 are thought to interact with each other through the association of the Ipaf amino-terminal CARD (caspase recruitment domain) and amino-terminal CARD of caspase-1.

Conjugate: Unconjugated
Immunogen: Ipaf antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of human Ipaf.
Buffer: Ipaf Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NLRC4 are readily searchable from our website. Different antibodies against the same target such as NLRC4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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