|The NKX2.5 col4a1 (Catalog #MBS850012) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NKX2.5 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s NKX2.5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA).
WB: 1/500 - 1/2000, ELISA: Propose dilution 1/10000
Not yet tested in other applications. Determining optimal working dilutions by titration test. Researchers should empirically determine the suitability of the NKX2.5 col4a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NKX2.5 col4a1 product has the following accession number(s) (GI #148536825) (NCBI Accession #NP_001836.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NKX2.5 Antibody with the following immunoassay(s):
Testing Data (Figure 1. Western blot analysis using NKX2.5 mouse mAb against full-length NKX2.5 (aa1-324)-hIgGFc transfected HEK293 cell lysate (1).)
Background: NKX2.5. NK2 transcription factor related, locus 5 (Drosophila), also known as CSX. It is a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants.
Immunogen: Purified recombinant fragment of human NKX2.5 expressed in E. Coli.
Species: Mouse IgG1. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NKX2.5 are readily searchable from our website. Different antibodies against the same target such as NKX2.5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Bone, Brain, Embryonic Tissue, Eye, Heart, Kidney, Liver, Muscle, Skin, Vascular tissues are correlated with this protein. The following patways have been known to be associated with this gene. NKX2.5 also interacts with the following gene(s): COL1A1, COL1A2, COL3A1, COL4A2, COL5A1, COL5A2, COL7A1. Angiopathy, Hereditary, With Nephropathy, Aneurysms, And Muscle Cramps, Brain Small Vessel Disease with Hemorrhage, Disease Models, Animal, Fibrosis, Heart Diseases, Hypertension, Hypertrophy, Inflammation, Kidney Diseases, Porencephaly are some of the diseases may be linked to NKX2.5 Antibody.