anti-NKX2-5 antibody product blog
Tags: Antibody; Polyclonal Antibody; CSX1/NKX2-5; anti-NKX2-5 antibody; NKX2-5;
The NKX2-5 nkx2-5 (Catalog #MBS422194) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CSX1/NKX2-5 Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Cow, Dog and may cross-react with other species as described in the data sheet. MyBioSource\'s CSX1/NKX2-5 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB).Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 45kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 34.9kDa according to NP_004378.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res.1996 Nov;79(5): 920-9.; PMID: 8888684). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the NKX2-5 nkx2-5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NKX2-5 nkx2-5 product has the following accession number(s) (GI #4758090) (NCBI Accession #NP_004378.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
PRAYSDPDPA KDPR.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-CSX1/NKX2-5 Antibody with the following immunoassay(s):
Western Blot (WB) ((0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunogen: Peptide with sequence C-PRAYSDPDPAKDPR, from the internal region of the protein sequence according to NP_004378.1; NP_001159647.1; NP_001159648.1.
Epitope: Internal region. Note: This antibody is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).
Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-8697. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NKX2-5 are readily searchable from our website. Different antibodies against the same target such as NKX2-5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. NKX2-5 also interacts with the following gene(s): BMP4, GATA4, HAND1, HAND2, MEF2A, MEF2C, SMAD4, SRF, TBX5. Brain, Embryonic Tissue, Heart, Intestine, Liver, Lung, Ovary, Spleen, Uncharacterized Tissue tissues are correlated with this protein.