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anti-NGF antibody product blog

Posted on 2014-05-10 01:29:14 by mybiosource_staff
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Tags: Antibody; NGF; Polyclonal Antibody; anti-NGF antibody;
The NGF shh (Catalog #MBS857191) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NGF Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NGF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF).
WB: 1:500-1:1000
IF: 1:20-1:50. Researchers should empirically determine the suitability of the NGF shh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The NGF shh product has the following accession number(s) (GI #4506939) (NCBI Accession #NP_000184.1) (Uniprot Accession #Q15465). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NGF Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of mouse livercell line, using SHH antibody.)
Western Blot (WB) NGF.

Hedgehog proteins (Hh) are secreted signaling proteins that play many roles during animal development. Aberrant Hh signaling activity can be associated with numerous birth defects and uncontrolled Hh pathway activation is linked to the development of several types of cancers (1-2). The three identified vertebrate Hh genes are Sonic (Shh), Indian (Ihh) and Desert (Dhh), all of which have distinct as well as overlapping roles (3-5). Hh proteins are synthesized as 45 kDa precursors that undergo auto-cleavage to generate a 19 kDa amino-terminal peptide (Hh-N) and a carboxy-terminal peptide (Hh-C). The amino-terminal peptide becomes covalently attached to a cholesterol molecule at its carboxy terminus and acetylated at its amino terminus. This doubly modified Hh-N peptide is released from cells and responsible for all known Hedgehog signaling activity (6).

Immunogen: A synthetic peptide of human SHH
Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NGF are readily searchable from our website. Different antibodies against the same target such as NGF may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. NGF also interacts with the following gene(s): BMP2, BMP4, GAS1, GLI1, GLI2, GLI3, GPC3, HHIP, PTCH1, PTCH2. The following patways have been known to be associated with this gene. Brain, Intestine, Liver, Lung, Uncharacterized Tissue tissues are correlated with this protein.
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