|The NFYB nfyb (Catalog #MBS120069) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human NFYB reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s NFYB can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the NFYB nfyb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NFYB nfyb product has the following accession number(s) (GI #45597173) (NCBI Accession #NP_006157.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse monoclonal antibody Anti-Human NFYB with the following immunoassay(s):
Quality Control (Left: Western blot analysis of immunized recombinant protein, using anti-NFYB monoclonal antibody.)
Immunocytochemistry (ICC) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-NFYB mAb. [Lot No.NFYBA7-2])
The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [NCBI Entrez Gene Summary].
Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Sterility: Filtered through a 0.22 um membrane. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NFYB are readily searchable from our website. Different antibodies against the same target such as NFYB may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. NFYB also interacts with the following gene(s): CIITA, EP300, MYC, NFYA, NFYC, SP1.