|The NCSTN ncstn (Catalog #MBS200143) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Nicastrin antibody (clone AT3B2) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Nicastrin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB) (Tissue).
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1000. Recommended starting dilution is 1:500. Researchers should empirically determine the suitability of the NCSTN ncstn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NCSTN ncstn product has the following accession number(s) (GI #24638433) (NCBI Accession #NP_056146). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Nicastrin antibody (clone AT3B2) with the following immunoassay(s):
Western Blot (WB) (The extracts of Mouse brain (80ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nicastrin (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Nicastrin is a large protein that is a component of an enzyme called gamma-secretase, which is lodged in the cell\'s membrane. Nicastrin protein is physically associated with presenilin and plays an important role in stabilization and correct localization of presenilin to the membrane-bound gamma-secretase complex. Nicastrin also serves as a docking site for gamma-secretase substrates such as APP and Notch, directly binding to them and presenting them properly to gamma-secretase to ensure the correct cleavage process.
Antigen Species: Human
Clone: Anti-human Nicastrin mAb, clone AT3B2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Nicastrin protein.
Immunogen: Recombinant human Nicastrin (34-153aa) purified from E Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NCSTN are readily searchable from our website. Different antibodies against the same target such as NCSTN may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.