anti-NCAM1 antibody product blog
Tags: Antibody; Polyclonal Antibody; NCAM1; anti-NCAM1 antibody;
The NCAM1 ncam1 (Catalog #MBS125304) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCAM1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NCAM1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS).WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
FC: 1:20 - 1:100. Researchers should empirically determine the suitability of the NCAM1 ncam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NCAM1 ncam1 product has the following accession number(s) (GI #205830665) (NCBI Accession #P13591.3) (Uniprot Accession #P13591). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NCAM1 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of mouse brain, using NCAM1 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.)
Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurones, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms\' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modiļ¬ed by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described.
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human NCAM1
Calculated Molecular Weight: 95kD. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NCAM1 are readily searchable from our website. Different antibodies against the same target such as NCAM1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.