anti-NANOG antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-NANOG antibody; NANOG;
The NANOG nanog (Catalog #MBS150440) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NANOG Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NANOG can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).NANOG antibody can be used for detection of NANOG by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the NANOG nanog for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NANOG nanog product has the following accession number(s) (GI #119609057) (NCBI Accession #EAW88651) (Uniprot Accession #Q9H9S0). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NANOG Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of NANOG in human spleen tissue lysate with NANOG antibody at (A) 1 and (B) 2 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of NANOG in human spleen tissue with NANOG antibody at 5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of NANOG in Human Spleen cells with NANOG antibody at 20 μg/mL.)
NANOG Antibody: Expression of NANOG is required for the maintenance of pluripotency in epiblast and embryonic stem (ES) cells as well as for the ability to maintain ES self-renewal independently of LIF/Stat3. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
Conjugate: Unconjugated
Immunogen: NANOG antibody was raised against a 19 amino acid synthetic peptide near the center of human NANOG.
Buffer: NANOG Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NANOG are readily searchable from our website. Different antibodies against the same target such as NANOG may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.