anti-MYH9 antibody product blog
Tags: Antibody; Polyclonal Antibody; MYH9; anti-MYH9 antibody;
The MYH9 myh9 (Catalog #MBS125840) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MYH9 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s MYH9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).WB: 1:500 - 1:1000
IHC: 1:50 - 1:100. Researchers should empirically determine the suitability of the MYH9 myh9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MYH9 myh9 product has the following accession number(s) (GI #6166599) (NCBI Accession #P35579.4) (Uniprot Accession #P35579). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MYH9 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using MYH9 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human esophagus using MYH9 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human stomach using MYH9 antibody at dilution of 1:100 (40x lens).)
Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7).
Species: Human
Immunogen: Recombinant Protein. Immunogen: Fusion protein of human MYH9
Calculated Molecular Weight: 227kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MYH9 are readily searchable from our website. Different antibodies against the same target such as MYH9 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.