anti-MYD88 antibody product blog
Tags: Antibody; Polyclonal Antibody; MyD88; anti-MYD88 antibody;
The MYD88 myd88 (Catalog #MBS420798) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MYD88 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. MyBioSource\'s MYD88 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA).Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Tonsil shows staining of clusters of cells some of which are likely plasma cells. Recommended concentration, 4-6ug/ml.
Western Blot: Approx 33kDa band observed in HumanThymus lysates (calculated MW of 33.2kDa according to NP_002459.1). Recommended concentration: 0.1-0.3ug/ml.
Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant MYD88 captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml. Researchers should empirically determine the suitability of the MYD88 myd88 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MYD88 myd88 product has the following accession number(s) (GI #289546503) (NCBI Accession #NP_001166038.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
IKYKAMKKEF P.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-MYD88 Antibody with the following immunoassay(s):
Western Blot (WB) (Staining (0.2ug/ml) of human thymus lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Immunohistochemistry (IHC) ((4ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not been obtained after antigen retireval at pH6 with this batch of antibody.)
Testing Data ((5ug/ml) as the reporter with as the capture rabbit antibody (5ug/ml).)
Immunogen: Peptide with sequence C-IKYKAMKKEFP, from the internal region of the protein sequence according to NP_001166038.1; NP_002459.2; NP_001166039.1.
Epitope: Internal region. Note: This antibody is expected to recognize reported isoforms 1, 2 and 3 (NP_001166038.1; NP_002459.2; NP_001166039.1). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MYD88 are readily searchable from our website. Different antibodies against the same target such as MYD88 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.