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anti-Mmp9 antibody product blog

Posted on 2015-06-29 17:49:57 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; MMP9; anti-MMP9 antibody; Gelatin, Skin;
The Mmp9 mmp9 (Catalog #MBS625052) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Gelatin, Skin reacts with Bovine and may cross-react with other species as described in the data sheet. MyBioSource\'s Gelatin, Skin can be used in a range of immunoassay formats including, but not limited to, Suitable for use in ELISA. Other applications not tested.
Dilution: ELISA: 1:1000-1:10,000; Coat gelatin antigen at 5-10ug/ml.

Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Mmp9 mmp9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The Mmp9 mmp9 product has the following accession number(s) (GI #1173506) (NCBI Accession #AAA90911.1) (Uniprot Accession #P50282). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular weights, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue and Type B gelatin is derived from lime-cured tissue. The charge on a gelatin molecule and its isoelectric point are primarily due to the carboxyl amino and guanidino groups on the side chains. Type A gelatin has 78-80 millimoles of free carboxyl groups per 100g of protein and a pI of 7.0-9.0; type B has 100-115 millimoles of free carboxyl groups per 100g of protein and a pI of 4.7-5.2. The pH of a 1.5% solution at 25C is 3.8-5.5 for Type An and 5.0-7.5 for Type B. Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to help stabilize Taq DNA polymerase, and use as a blocking reagent in Western Blot, ELISA, and Immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media for species differentiation. Additionally, as a biocompatible polymer, gelatin has been used as a delivery vehicle for the release of bioactive molecules and in the generation of scaffolds for tissue engineering applications. Gelatin proteins are also used as additives in purified, recombinant proteins and antibodies. Animals or humans exposed to gelatins may invoke antibody response. Often the gelatin must be removed prior to such applications. Antibodies to gelatins were produced to detect the presence of gelatins in biolgocial samples and or to verify their removal during bioprocess development.

Mmp9 mmp9

Immunogen: Gelatin, bovine skin Type B. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Mmp9 are readily searchable from our website. Different antibodies against the same target such as Mmp9 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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