anti-MKP-1 antibody product blog
Tags: Antibody; Polyclonal Antibody; MKP-1 / DUSP1; anti-MKP-1 antibody; MKP-1;
The MKP-1 dusp1 (Catalog #MBS242187) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human MKP-1 / DUSP1 reacts with Bat, Gibbon, Bovine, Chicken, Dog, Gorilla, Horse, Human, Monkey, Pig, RabbitPredicted Reactivity: Rat, Hamster, Xenopus, Zebrafish (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. MyBioSource\'s MKP-1 / DUSP1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA).
ELISA (1:32000), IHC-P (3.75 ug/ml), WB (0.3 - 1 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the MKP-1 dusp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MKP-1 dusp1 product has the following accession number(s) (GI #4758204) (NCBI Accession #NP_004408.1) (Uniprot Accession #P28562). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat Polyclonal to Human MKP-1 / DUSP1 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-DUSP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 75 ug/ml.)
Western Blot (WB) (Antibody (0.3 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
DUSP1 is a dual specificity MAP kinase phosphatase. DUSP1 has been shown to inactivate MAP kinases in a variety of tissues by dephosphorylating critical tyrosine and threonine residues. It has also been implicated as a dual serine-threonine phosphatase, inactivating the kinases ERK and JNK/SAPK which are involved in neuronal survival and neuronal cell death following injury and degenerative stimuli. DUSP1 is induced by oxidative stress, heat shock and growth factors.
Target Species: Human
Immunogen Description: Synthetic peptide SYLQSPITTSPSC from the C-terminus of human DUSP1 (NP_004408.1). Immunogen Type: Synthetic peptide
Immunogen: MKP-1 / DUSP1 antibody was raised against synthetic peptide SYLQSPITTSPSC from the C-terminus of human DUSP1 (NP_004408.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Lizard (100%); Rat, Hamster, Xenopus, Zebrafish (92%); Mouse, Opossum, Stickleback, Pufferfish (85%).
Antigen Modification: C-Terminus. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MKP-1 are readily searchable from our website. Different antibodies against the same target such as MKP-1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.