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anti-MEIS2 antibody product blog

Posted on 2015-05-11 02:02:47 by mybiosource_staff
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Tags: Antibody; MEIS2; anti-MEIS2 antibody; Polyclonal Antibody;
The MEIS2 meis2 (Catalog #MBS422372) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MEIS2 Antibody reacts with Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Dog, Cow and may cross-react with other species as described in the data sheet. MyBioSource\'s MEIS2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB).
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 50kDa bands observed in Rat Brain and Rat Thymus lysates, 48kDa band in Mouse Brain and 40kDa in Mouse Thymus (calculated MW of 51.8kDa according to Human NP_733775.1 and Mouse NP_001153040.1, and of 43.7kDa according to Mouse NP_001153039.1 and NP_001153041.1). Recommended concentration: 0.1-0.3 ug/ml. Researchers should empirically determine the suitability of the MEIS2 meis2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The MEIS2 meis2 product has the following accession number(s) (GI #24762244) (NCBI Accession #NP_733777.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-MEIS2 Antibody with the following immunoassay(s):
Western Blot (WB) ((0.1ug/ml) staining of Mouse Brain (lane A), Rat Brain (lane B), Mouse Thymus (lane C) and Rat Thymus (lane D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB) MEIS2.

Immunogen: Peptide with sequence C-QSNRAGFLLDPSVSQ, from the internal region of the protein sequence according to NP_733777.1; NP_733775.1; NP_758526.1.
Epitope: Internal region. Note: This antibody is expected to recognize reported Human isoforms a (NP_733777.1), c (NP_733775.1), and g (NP_758526.1), and reported Mouse isoforms 3, 4 and 5 (NP_001153039.1, NP_001153040.1, NP_001153041.1). NB: The immunizing peptide shows no overlap with the reported PROVISIONAL Rat protein NP_001101228.1 but is does with UniprotKB entry D4A2T5. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MEIS2 are readily searchable from our website. Different antibodies against the same target such as MEIS2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. MEIS2 also interacts with the following gene(s): BMP2, DACH1, HOXA9, HOXB7, PAX3, PAX6, PBX1, TSHZ2.
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