anti-MED28 antibody product blog
Tags: Antibody; Polyclonal Antibody; MED28; anti-MED28 antibody;
The MED28 med28 (Catalog #MBS150807) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MED28 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s MED28 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).MED28 antibody can be used for detection of MED28 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the MED28 med28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MED28 med28 product has the following accession number(s) (GI #52851391) (NCBI Accession #NP_079481) (Uniprot Accession #Q9H204). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MED28 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of MED28 in human brain tissue lysate with MED28 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Immunohistochemistry (IHC) (Immunohistochemistry of MED28 in human brain tissue with MED28 antibody at 2.5 μg/mL.)
MED28 Antibody: The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED28, also known as Magicin. MED28 is expressed in many cell lines and tissues. It has been shown that a down-regulation of MED28 expression in NIH3T3 cells results in a significant induction of several genes associated with smooth muscle cell differentiation and conversely its over-expression represses expression of SMC genes. MED28 can also form a ternary complex with Grb2 and Merlin, the neurofibromatosis 2 tumor suppressor protein, indicating that MED28 may play a role in Merlin\'s tumor suppressive activity. MED28 has also been recently identified as an HIV dependency factor (HDF), suggesting that MED28 may be an important drug target in HIV treatment. At least two isoforms of MED28 are known to exist.
Conjugate: Unconjugated
Immunogen: MED28 antibody was raised against a 17 amino acid synthetic peptide from near the center of human MED28.
Buffer: MED28 Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MED28 are readily searchable from our website. Different antibodies against the same target such as MED28 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.