anti-MECOM antibody product blog
Tags: Antibody; Polyclonal Antibody; EVI1/AML1; anti-MECOM antibody; MECOM;
The MECOM mecom (Catalog #MBS422330) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-EVI1/AML1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. MyBioSource\'s EVI1/AML1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB).Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 170kDa band observed in lysates of cell lines A549 and HepG2 (calculated MW of 126kDa according to NP_001098547.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the MECOM mecom for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MECOM mecom product has the following accession number(s) (GI #255683379) (NCBI Accession #NP_001098547.3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
DKESLHSTSH.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-EVI1/AML1 Antibody with the following immunoassay(s):
Western Blot (WB) ((2ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunogen: Peptide with sequence C-DKESLHSTSH, from the internal region of the protein sequence according to NP_001098547.3; NP_005232.2; NP_004982.2; NP_001157471.1; NP_001157472.1.
Epitope: Internal region. Note: This antibody is expected to recognize all reported isoforms (NP_001098547.3; NP_005232.2; NP_004982.2; NP_001157471.1; NP_001157472.1). Reported variants represent identical protein: NP_001098548.2, NP_005232.2. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MECOM are readily searchable from our website. Different antibodies against the same target such as MECOM may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.