anti-MAPK1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-MAPK1 antibody; MAPK1; MAPK1 / ERK2 / p42;
The MAPK1 mapk1 (Catalog #MBS241457) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal (IgG) to Rat MAPK1 / ERK2 / p42 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s MAPK1 / ERK2 / p42 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP).IHC-P (1:50), IP, WB
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the MAPK1 mapk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MAPK1 mapk1 product has the following accession number(s) (GI #66932916) (NCBI Accession #NP_002736.3) (Uniprot Accession #P28482). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit Polyclonal (IgG) to Rat MAPK1 / ERK2 / p42 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-MAPK1 / ERK antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)
Western Blot (WB) (Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-ERK2 polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.)
ERK2, a MAPK type protein kinase, is a member of the Ras MAPK pathway. This pathway modulates the activity of transcription factors, structural proteins and other protein kinases, leading to biological responses such as cell proliferation and differentiation. Inactive ERK2 associates with MAPK/ERK kinases (MEK) 1 and 2 in the cytosol. Upon stimulation, MEK1/2 phosphorylates and activates ERK2 causing dissociation of the MEK/ERK complex.
Target Species: Rat
Immunogen Description: Synthetic peptide from rat MAPK1 / ERK. Immunogen Type: Synthetic peptide
Immunogen: MAPK1 / ERK2 / p42 antibody was raised against synthetic peptide from rat MAPK1 / ERK.
Antigen Modification: C-Terminus. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MAPK1 are readily searchable from our website. Different antibodies against the same target such as MAPK1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.