anti-MAPK1 antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-MAPK1 antibody; MAPK1;
The MAPK1 mapk1 (Catalog #MBS200186) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MAPK1 antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s MAPK1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF).WB/IF: 1:500 ~ 3000.
Recommended starting dilution is 1:500. Researchers should empirically determine the suitability of the MAPK1 mapk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MAPK1 mapk1 product has the following accession number(s) (GI #20986531) (NCBI Accession #NP_620407) (Uniprot Accession #P28482). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MAPK1 antibody with the following immunoassay(s):
Western Blot (WB) (Cell lysates of HepG2 (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK1 (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Immunofluorescence (IF) (Immunofluorescence of human MCF7 cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human MAPK1 antibody (1:500) with Alexa 488 (Green).)
Mitogen-activated protein kinases (MAPKs) are reported to be critical regulatory factors for the growth andmigration of various cell types including vascular smooth muscle cells (VSMCs). It has also been reported that the activation of the MAP kinase family, extracellular signal regulated kinases 1/2 (ERK1/2), and c-Jun N-terminal kinase (JNK) via Src activation is important for AII-induced migration of VSMCs. MAPKs are intracellular signal-transduction pathways that have been shown to play a central role in the development of injury following ischemia in the brain and heart. MAPKs regulate gene expression, which is important in cell injury/repair and proliferation/differentiation.
Antigen Species: Human
Immunogen: Recombinant human MAPK1 (1-360) purified from E Coli. Clone Note: Anti-human MAPK1 mAb, clone AT1A4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MAPK1 protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MAPK1 are readily searchable from our website. Different antibodies against the same target such as MAPK1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.