|The MAD2L2 mad2l2 (Catalog #MBS240549) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human MAD2B / REV7 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s MAD2B / REV7 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA).
ELISA (1:7500 - 1:30000), IF, IHC-P (10 ug/ml), WB (1:500 - 1:2000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib.. Researchers should empirically determine the suitability of the MAD2L2 mad2l2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MAD2L2 mad2l2 product has the following accession number(s) (GI #187960073) (NCBI Accession #NP_006332.3) (Uniprot Accession #Q9UI95). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit Polyclonal to Human MAD2B / REV7 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-MAD2L2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Western Blot (WB) (Anti-MAD2L2 Antibody - Western Blot. Affinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a ~20 kD band corresponding to human MAD2L2 (predicted molecular weight is 24 kD). Approximately 20 ug of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.)
Target Species: Human
Immunogen Description: Synthetic peptide from human MAD2L2. Immunogen Type: Synthetic peptide
Immunogen: MAD2B / REV7 antibody was raised against synthetic peptide from human MAD2L2.
Antigen Modification: aa3-14. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MAD2L2 are readily searchable from our website. Different antibodies against the same target such as MAD2L2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.