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anti-MAD2L1 antibody product blog

Posted on 2014-03-02 19:17:05 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; MAD2L1; MAD2L1 / MAD2; anti-MAD2L1 antibody;
The MAD2L1 mad2l1 (Catalog #MBS240551) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human MAD2L1 / MAD2 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s MAD2L1 / MAD2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA).
ELISA (1:2000 - 1:10000), IF, IHC-P (2.5 ug/ml), WB (1:500 - 1:2000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib.. Researchers should empirically determine the suitability of the MAD2L1 mad2l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The MAD2L1 mad2l1 product has the following accession number(s) (GI #4505067) (NCBI Accession #NP_002349.1) (Uniprot Accession #Q13257). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit Polyclonal to Human MAD2L1 / MAD2 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-MAD2L1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC) MAD2L1.

Western Blot (WB) (Anti-MAD2L1 Antibody - Western Blot. Western blot of Affinity Purified anti-MAD2L1 antibody shows detection of a predominant band at ~24 kD corresponding to MAD2L1 (arrowhead) present in Jurkat (lane 1) and HeLa (lane 2) whole cell lysates using the 800 nm channel (green). The identity of the higher molecular weight bands is unknown, although they may represent complexes of MAD2L1 with related binding proteins. Specific band reactivity is blocked when the antibody is pre-incubated with immunizing peptide (lanes 4 and 5 respectively) which completely blocks antibody staining. ~35 ug of lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1200. Incubation was 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MXHu (for 45 min at room temperature. Molecular weight markers were used for size comparison using the 700 nm channel (lane 3). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.)
Western Blot (WB) MAD2L1.

Target Species: Human
Immunogen Description: Synthetic peptide from human MAD2L1. Immunogen Type: Synthetic peptide
Immunogen: MAD2L1 / MAD2 antibody was raised against synthetic peptide from human MAD2L1.
Antigen Modification: aa3-13.
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