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anti-LIME1 antibody product blog

Posted on 2015-05-26 15:14:37 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; Lime1; anti-LIME1 antibody;
The Lime1 lime1 (Catalog #MBS421065) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Lime1 (mouse) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Lime1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB).
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 32kDa band observed in Mouse Heart lysates (predicted MW of 31kDa according to NP_076173.1). Recommended for use at 0.5-2ug/ml. Researchers should empirically determine the suitability of the Lime1 lime1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The Lime1 lime1 product has the following accession number(s) (GI #12963783) (NCBI Accession #NP_076173.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-Lime1 (mouse) Antibody with the following immunoassay(s):
Western Blot (WB) (Staining (1ug/ml) of Mouse Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB) Lime1.

Immunogen: Peptide with sequence C-ENVYESIKEMGL, from the C Terminus of the protein sequence according to NP_076173.1.
Epitope: C Terminus. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Lime1 are readily searchable from our website. Different antibodies against the same target such as Lime1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Lung tissues are correlated with this protein.
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