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anti-LGP2 antibody product blog

Posted on 2014-02-08 14:45:08 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; LGP2; anti-LGP2 antibody;
The LGP2 dhx58 (Catalog #MBS223713) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s LGP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
Western Blot: This item detects a band of approximately 41 kDa in MDA-MB-361 cell lysate.
Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml. Researchers should empirically determine the suitability of the LGP2 dhx58 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The LGP2 dhx58 product has the following accession number(s) (GI #149408122) (NCBI Accession #NP_077024.2) (Uniprot Accession #Q96C10). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RABBIT ANTI LGP2 (N-TERMINAL) with the following immunoassay(s):
Testing Data #1 (Western blot analysis of whole cell lysate from MDA-MB-361 human breast adenocarcinoma cells probed with Rabbit anti Human LGP2 (MBS224002) at 0.5(A) and 1(B) ug/ml)
Testing Data #1 LGP2.

Rabbit anti LGP2 (N-Terminal) antibody recognizes human Probable ATP-dependent helicase LGP2 (LGP2), also known as DHX58, a 768aa cytoplasmic protein belonging to the Helicase family. LGP2 is a negative regulator of host innate immune defense against viruses. The repressor domain of LGP2 interacts with RIG-I and negatively regulates RIG-I mediated signaling. LGP2 binds to dsRNA produced during viral replication, in particuliar HCV RNA.

Perservative Stabilisers: 0.02% Sodium Azide (NaN3). Immunogen: Peptide corresponding to a 14 amino acid sequence from near the amino terminus of human LGP2.
Buffer Solution: Phosphate buffered saline
Antiserum Preparation: Antisera to human LGP2 (N-Terminal) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing LGP2 are readily searchable from our website. Different antibodies against the same target such as LGP2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Blood, Brain, Intestine, Kidney, Liver, Lung, Mouth, Pancreas, Spleen, Vascular tissues are correlated with this protein. LGP2 also interacts with the following gene(s): DDX58, EIF2AK2, IFIH1, IRF7, ISG15, RSAD2.
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