anti-LGALS14 antibody product blog
Tags: Antibody; Monoclonal Antibody; Galectin-14; LGALS14; anti-LGALS14 antibody;
The LGALS14 lgals14 (Catalog #MBS605811) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Galectin-14 (LGALS14 lectin, galactoside-binding, soluble, Charcot-Leyden crystal protein 2, CLC2, MGC22235, Placental protein 13-like, PPL13) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Galectin-14 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).Suitable for use in Western Blot.
Dilution: Western Blot: 2ug/ml with the appropriate secondary reagents to detect human Galectin-14 in cell or tissue extracts under non-reducing conditions only. Lysates were prepared from primary human eosinophils in non-reducing sample buffer, resolved by SDS-PAGE 10ug total protein/lane), and transferred to an Blotting Buffer Blocking Solution Antibody Solution 25mM Tris, pH 7.4 5% nonfat dry milk 5% nonfat dry milk 0.15M NaCl in Blotting Buffer in Blotting Buffer 0.05% Tween 20.
Direct ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect human Galectin-14. The detection limit for rhGalectin-14 is approximately 3ng/well. Researchers should empirically determine the suitability of the LGALS14 lgals14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The LGALS14 lgals14 product has the following accession number(s) (GI #237664147) (Uniprot Accession #Q8TCE9). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Galectin-14, also known as placental protein 13-like (PPL13), belongs the galectin family of carbohydrate binding proteins. It is expressed intracellularly in placenta and eosinophils and is released by eosinophils following allergen stimulation. Galectin-14 may be involved in the development of allergic inflammation.
Immunogen: Purified, E. coli-derived, recombinant human Galectin-14 (rhGalectin-14; aa 1-139; Accession# Q8TCE9). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing LGALS14 are readily searchable from our website. Different antibodies against the same target such as LGALS14 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.