anti-Leptospira antibody product blog
Tags: Antibody; Polyclonal Antibody; Leptospira; anti-Leptospira antibody;
The Leptospira n/a (Catalog #MBS324031) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Leptospira can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunoblotting, ELISA (IEA).Working strength must be determined by the user, but the recommended ranges to start are:
IHC/Blotting: 1:20-1:200
EIA: 1:200-1:1000. Researchers should empirically determine the suitability of the Leptospira n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods developed by ViroStat. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3. This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/ml) and preserved with 0.002% thimerosal. This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for for enzyme immunoassays. Recommended short term (<6 months) storage is liquid at 2-8 degree C. For longer term storage, aliquot and freeze.Unit size is 1.0 ml.NOTE: sodium azide is a potent inhibitor of peroxidase !.
Immunogen: Whole organism
Antiserum Characterization: (neat)
Titer: >1:500 in direct FA. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Leptospira are readily searchable from our website. Different antibodies against the same target such as Leptospira may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.