anti-Lep antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-lep antibody; lep; Leptin Clone 2A4-A1;
The Lep lep (Catalog #MBS350023) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-human Leptin (92-145) cyclized Monoclonal Antibody Clone 2A4-A1 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Leptin Clone 2A4-A1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescent Staining of Cells.Immunofluorescent: This monoclonal antibody has been found to stain specifically human adipocytes at a concentration of 0.25 ug/ml. The ability of this monoclonal antibody to bind to leptin in adipocytes was examined in cells fixed with neutral buffered formalin. The fixed cells were incubated for 20 min with 4% normal goat serum. reacted for 60 minutes with the diluted mouse monoclonal antibody. and then with FITC-conjugated goat anti-mouse IgG. The immunofluorescent staining pattern was observed using epifluorescent microscopy. Researchers should empirically determine the suitability of the Lep lep for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Lep lep product has the following accession number(s) (GI #6981148) (NCBI Accession #NP_037208.1) (Uniprot Accession #P50596). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
This IgG fraction was isolated from ascites fluid and contains mouse monoclonal antibody 2A4-A1 raised against synthetic human leptin (92-145)cyclized. The 50 ?gm of purified IgG fraction has been packaged in 0.10 ml of 10mg/ml BSA in PBS as carrier protein. This monoclonal antibody has been shown to bind to the carboxyl terminal region of the protein and has been found to bind to intact leptin specifically in ELISAs and by immunocytochemistry. It has been found to be mouse IgG2A by isotyping.
In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Lep are readily searchable from our website. Different antibodies against the same target such as Lep may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Brain, Lung tissues are correlated with this protein. Cardiovascular Diseases, Diabetes Mellitus, Hypertension, Inflammation, Insulin Resistance, Kidney disorder involving deposition of calcium and oxalate or phosphate in the renal tubules, Neoplasms, Obesity, Pain, Weight Gain are some of the diseases may be linked to Anti-human Leptin (92-145) cyclized Monoclonal Antibody Clone 2A4-A1.