anti-LAMC2 antibody product blog
Tags: Antibody; Polyclonal Antibody; LAMC2; anti-LAMC2 antibody;
The LAMC2 lamc2 (Catalog #MBS175527) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-LAMC2 Antibody reacts with Cross-Reactivity: No cross reactivity with other proteinsReacts with: Human, Rat
Predict to work with: Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s LAMC2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin.
Application details contain suggested dilutions. End user should optimize the final workingconcentrations.
Immunohistochemistry(Paraffin-embedded Section):
Dilution: 0.5-1ug/ml
Tested Species: Human, Rat
Predicted Species: Mouse
Antigen Retrieval: By Heat
Western blot:
Dilution:0.1-0.5ug/ml
Tested Species: Human, Rat
Predicted Species: Mouse
Tested Species: In-house tested species with positivie results.
Predicted Species: Species predicted to be fit for the product based on sequence similaritites.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/praffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the LAMC2 lamc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The LAMC2 lamc2 product has the following accession number(s) (GI #90185107) (NCBI Accession #Q13753.2) (Uniprot Accession #Q13753). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-LAMC2 Antibody with the following immunoassay(s):
Western Blot (WB) (Anti-LAMC2 antibody, MBS175527, Western blotting
All lanes: Anti LAMC2 (MBS175527) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 131KD
Observed bind size: 131KD )
Immunohistochemistry (IHC) (Anti-LAMC2 antibody, MBS175527, IHC(P)
IHC(P): Human Lung Cancer Tissue )
Rabbit IgG polyclonal antibody for Laminin subunitgamma-2(LAMC2) detection. Tested with WB, IHC-P inHuman;Mouse;Rat.
Immunogen: A synthetic peptide corresponding to a sequence at the C-terminusof human LAMC2(1160-1180aa ETSIDGILADVKNLENIRDNL),identical to the related rat and mouse sequences.
Gene Name: LAMC2
Protein Name: Laminin subunit gamma-2
Protein Function: Binding to cells via a high affinity receptor, lamininis thought tomediate the attachment, migration and organizationof cells intotissues during embryonic development by interactingwith otherextracellular matrix components. Ladsin exerts cell-scatteringactivity toward a wide variety of cells, includingepithelial,endothelial, and fibroblastic cells.
Tissue Specificity: The large variant is expressed only inspecific epithelial cells ofembryonic and neonatal tissues. In17-week old embryo the smallvariant is found in cerebral cortex,lung, and distal tubes of kidney,but not in epithelia except fordistal tubuli.