anti-KRT5 antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-KRT5 antibody; KRT5; KRT5 / CK5 / Cytokeratin 5;
The KRT5 krt5 (Catalog #MBS246311) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 1E1] (IgG1) to Human KRT5 / CK5 / Cytokeratin 5 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s KRT5 / CK5 / Cytokeratin 5 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA).ELISA (1:10000), IHC-P (1:200 - 1:400), WB (1:500 - 1:2000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the KRT5 krt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The KRT5 krt5 product has the following accession number(s) (GI #119395754) (NCBI Accession #NP_000415.2) (Uniprot Accession #P13647). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Monoclonal [clone 1E1] (IgG1) to Human KRT5 / CK5 / Cytokeratin 5 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-KRT5 / Cytokeratin 5 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)
Immunohistochemistry (IHC) (Anti-KRT5 / Cytokeratin 5 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)
Immunohistochemistry (IHC) (Anti-KRT5 / Cytokeratin 5 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)
Western Blot (WB) (Western blot of anti-CK5 monoclonal antibody against HeLa cell lysate(1).)
KRT5 encodes the type II keratin 5, an intermediate filament chain expressed largely in stratified squamous epithelia. Mutations in KRT5 cause epidermolysis bullosa simplex.
Target Species: Human
Immunogen Description: Purified recombinant fragment of Cytokeratin 5 expressed in E Coli. Immunogen Type: Recombinant protein
Immunogen: KRT5 / CK5 / Cytokeratin 5 antibody was raised against purified recombinant fragment of Cytokeratin 5 expressed in E Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing KRT5 are readily searchable from our website. Different antibodies against the same target such as KRT5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.