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anti-KRT18 antibody product blog

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Posted on 2014-01-27 07:36:23 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; anti-KRT18 antibody; KRT18; Cytokeratin Peptide 18;
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The KRT18 krt18 (Catalog #MBS175098) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cytokeratin Peptide 18 antibody (monoclonal) reacts with Human, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Cytokeratin Peptide 18 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin. Researchers should empirically determine the suitability of the KRT18 krt18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The KRT18 krt18 product has the following accession number(s) (GI #125083) (NCBI Accession #P05783.2) (Uniprot Accession #P05783). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Cytokeratin Peptide 18 antibody (monoclonal) with the following immunoassay(s):
Western Blot (WB) (Anti-Cytokeratin Peptide 18 antibody (monoclonal), MBS175098, Western blotting
WB: MCF-7 Cell Lysate )
Western Blot (WB) KRT18.

Immunohistochemistry (IHC) (Anti-Cytokeratin Peptide 18 antibody (monoclonal), MBS175098, IHC(P)
IHC(P): Human Mammary Cancer Tissue )
Immunohistochemistry (IHC) KRT18.

Immunohistochemistry (IHC) (Anti-Cytokeratin Peptide 18 antibody (monoclonal), MBS175098, IHC(P)
IHC(P): Human Rectal Cancer Tissue )
Immunohistochemistry (IHC) KRT18.

Immunohistochemistry (IHC) (Anti-Cytokeratin Peptide 18 antibody (monoclonal), MBS175098, IHC(P)
IHC(P): Human Mammary Cancer Tissue

)
Immunohistochemistry (IHC) KRT18.

Description: Mouse IgG monoclonal antibody for Cytokeratin Peptide 18, keratin 18 (KRT18) detection. Tested with WB, IHC-P, IHC-F in Human, rat. No cross reactivity with other proteins. Uniprot ID: P05783
Background: Intermediate filaments(IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18(KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis.

Immunogen: The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines. Contents: Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing KRT18 are readily searchable from our website. Different antibodies against the same target such as KRT18 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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