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anti-KPNA2 antibody product blog

Posted on 2013-08-29 18:48:37 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; KPNA2; anti-KPNA2 antibody; KPNA2/IPOA1;
The KPNA2 kpna2 (Catalog #MBS421014) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-KPNA2/IPOA1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. MyBioSource\'s KPNA2/IPOA1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF).
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 55kDa band observed in lysates of cell line HeLa (calculated MW of 57.9kDa according to NP_002257.1). Recommended for use at 1-3ug/ml.
Immunofluorescence: In 4% formaldahyde-fixed HeLa staining is mostly cytoplasmic, with some nuclear staining and clear nuclear pore staining. Researchers should empirically determine the suitability of the KPNA2 kpna2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The KPNA2 kpna2 product has the following accession number(s) (GI #4504897) (NCBI Accession #NP_002257.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-KPNA2/IPOA1 Antibody with the following immunoassay(s):
Western Blot (WB) (Staining (0.1ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB) KPNA2.

Immunofluorescence (IF) (Staining (top panel and green) of 4% formaldahyde-fixed HeLa with endogenously expressed HistonH2B-GFP fusion as DNA marker (mid panel and red). Data kindly provided by Francisco Iborra, MRC Mol. Haematol. Unit, Oxford.)
Immunofluorescence (IF) KPNA2.

Immunogen: Peptide with sequence C-QVQDGAPGTFNF, from the C Terminus of the protein sequence according to NP_002257.1.
Epitope: C Terminus. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing KPNA2 are readily searchable from our website. Different antibodies against the same target such as KPNA2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Blood, Brain, Embryonic Tissue, Heart, Liver, Lung, Lymph, Lymph Node, Muscle, Prostate tissues are correlated with this protein. KPNA2 also interacts with the following gene(s): KPNB1, NUP153, NUP62, RANBP2.
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