anti-KLF4 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-KLF4 antibody; KLF4; KLF4/GKLF;
The KLF4 klf4 (Catalog #MBS420312) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-KLF4/GKLF Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. MyBioSource\'s KLF4/GKLF can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB).Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Experiments gave strong bands at 75-90kDa and a faint 50-kDa band in Human Duodenal lysate after 0.1ug/ml antibody staining. These bands correspond to earlier findings in literature (Piccinni et al, J. Biol. Chem., Vol.279 (10), 9103-9114). All these bands were successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the KLF4 klf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The KLF4 klf4 product has the following accession number(s) (GI #33620777) (NCBI Accession #NP_004226.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
AVSDALLPSF ST.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-KLF4/GKLF Antibody with the following immunoassay(s):
Western Blot (WB) ((0.1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunogen: Peptide with sequence AVSDALLPSFST-C, from the N Terminus of the protein sequence according to NP_004226.2.
Epitope: N Terminus. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing KLF4 are readily searchable from our website. Different antibodies against the same target such as KLF4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Breast Neoplasms, Carcinoma, Squamous Cell, Cardiovascular Diseases, Disease Models, Animal, Heart Diseases, Inflammation, Liver Neoplasms, Necrosis, Neoplasms, Experimental, Nervous System Diseases are some of the diseases may be linked to Goat anti-KLF4/GKLF Antibody. Brain, Embryonic Tissue, Eye, Heart, Intestine, Kidney, Liver, Lung, Skin, Vascular tissues are correlated with this protein. The following patways have been known to be associated with this gene.