anti-KL antibody product blog
Tags: Antibody; Klotho; Monoclonal Antibody; anti-KL antibody;
The KL kl (Catalog #MBS603640) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Klotho (KL) reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Klotho can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC).Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry.
Dilution: Western Blot: 1-5ug/ml. Using Chemiluminescence technique.
ELISA: 1:10,000-1:100,000. Using 50-100 ng of control peptide/well.
Immunohistochemistry: 2-20ug/ml. Researchers should empirically determine the suitability of the KL kl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The KL kl product has the following accession number(s) (GI #2618596) (Uniprot Accession #Q9UEF7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Animal models of human aging, which display the characteristic diseases associated with aging, provide insight into the cause of such diseases. Recently a transgenic mouse model, called Klotho, with several premature aging phenotypes has been described. They display premature aging phenotypes such as Osteoporosis, age related skin changes, ectopic calcifications, atrophy of genital organs and thymus, emphysema and short life span (1). KL protein associated with the Klotho mutation is a 1014 amino acid long peptide. It has a putative signal sequence at its N-terminus and a single transmembrane domain near its C-terminus, which is postulated to anchor it to the membrane (2). The extracellular domain has two internal repeats, KL-1 and KL-2 which have 20-40% sequence identity to bacterial b-glucosidase of plants and animals and mammalian lactase glycosylceramidase suggesting a role for Klotho in sphingolipid metabolism (2). kl gene expression was observed to be tissue specific. Improvement of systemic aging phenotypes in kl/kl mice occurs even when the exogenous expression was limited to some organs, suggesting that KL associated aging is regulated through a humoral signaling pathway. KL has been reported to be localized on the cell surface when expressed on Cho cells (1). Human kl cDNA is expected to encode a protein of 1012 amino acids and has 86% homology to mouse kl. Alternative splicing produces two isoforms (a full length membrane bound and the secreted form).
Immunogen: Purified recombinant mouse klotho protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing KL are readily searchable from our website. Different antibodies against the same target such as KL may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.