anti-KIR2DL1 antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-KIR2DL1 antibody; KIR2DL1;
The KIR2DL1 kir2dl1 (Catalog #MBS200033) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KIR2DL1 antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s KIR2DL1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF).WB: 1:500 ~ 2,000.
Recommended starting dilution is 1:500. Researchers should empirically determine the suitability of the KIR2DL1 kir2dl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The KIR2DL1 kir2dl1 product has the following accession number(s) (GI #110611923) (NCBI Accession #NP_055033) (Uniprot Accession #P43626). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the KIR2DL1 antibody with the following immunoassay(s):
Western Blot (WB) (The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: Jurkat cell lysate Recombinant human protein KIR2DL1, KIR2DL3, KIR2DS4 and KIR2DL4 (each 50ng per well) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: KIR2DL1 recombinant protein Lane 2.: KIR2DL3 recombinant protein Lane 3.: KIR2DS4 recombinant protein Lane 4.: KIR2DL4 recombinant protein)
Immunofluorescence (IF) (ICC/IF analysis of KIR2DL1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KIR2DL1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
The kIR family consists of transmembrane glycoproteins of the Ig superfamily expressed on human Nk cells and a subset of human T cells which they are involved in recognition of either MHC class I molecules or unknown ligand on target cells and inhibit cytotoxic activities.
Antigen Species: Human
Immunogen: Recombinant human kIR2DL1 (23-223 aa) purified from E. coli. Clone Note: Anti-human kIR2DL1 mAb, clone 2F9, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human kIR2DL1 protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing KIR2DL1 are readily searchable from our website. Different antibodies against the same target such as KIR2DL1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.