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anti-KDM1 antibody product blog

Posted on 2013-12-06 00:44:23 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; LSD1; anti-KDM1 antibody; KDM1;
The KDM1 kdm1a (Catalog #MBS151105) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LSD1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s LSD1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).
LSD1 antibody can be used for the detection of LSD1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the KDM1 kdm1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The KDM1 kdm1a product has the following accession number(s) (GI #58761544) (NCBI Accession #NP_001009999) (Uniprot Accession #O60341). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the LSD1 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of LSD1 in P815 cell lysate with LSD1 antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB) KDM1.

Immunohistochemistry (IHC) (Immunohistochemistry of LSD1 in human small intestine tissue with LSD1 antibody at 2 μg/mL.)
Immunohistochemistry (IHC) KDM1.

Immunofluorescence (IF) (Immunofluorescence of LSD1 in Human Small Intestine cells with LSD1 antibody at 20 μg/mL.)
Immunofluorescence (IF) KDM1.

LSD1 Antibody: Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction. Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription. LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation. Experiments have shown that CoREST, a SANT domain-containing corepressor acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein, inhibits CoREST/LSD1 activity in vitro. LSD1-mediated histone demethylation thus may have significant effects on gene expression.

Conjugate: Unconjugated
Immunogen: LSD1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human LSD1.
Buffer: LSD1 Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing KDM1 are readily searchable from our website. Different antibodies against the same target such as KDM1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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