|The JAK2 jak2 (Catalog #MBS395346) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The JAK2 reacts with Humans, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s JAK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
Antibody can be used for Western Blotting (1-5ug/ml)
Optimal concentration should be evaluated by serial dilutions. Researchers should empirically determine the suitability of the JAK2 jak2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The JAK2 jak2 product has the following accession number(s) (GI #4826776) (NCBI Accession #NP_004963.1) (Uniprot Accession #O60674). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the JAK2 with the following immunoassay(s):
Western Blot/Blotting (Western blot analysis using JAK2 antibody, clone 691R5 on Jurkat lysate. Antibody used at 1 ug/ml and incubated for 2 hours at room temperature.)
Janus Activating Kinase 2 (JAK2) is a 130 kDa tyrosine kinase involved in cytoplasmic signal transduction. Ligand binding to a variety of cell surface receptors (e.g., cytokine, growth factor, GPCRs) leads to an association of those receptors with JAK proteins, which are then activated via phosphorylation on tyrosines 1007 and 1008 in the kinase activation loop. Activated JAK proteins phosphorylate and activate STAT (signal transducers and activators of transcription) proteins, which then dimerize and translocate to the nucleus. Once in the nucleus, STAT proteins bind to DNA and modify the transcription of various genes, which can lead to various responses such as cell proliferation, cell survival and differentiation.
Immunogen: Recombinant full length human JAK2 protein expressed in E.coli. Positive Control: Human Jurkat cells, mouse 3T3L1 cells and rat L6 cells. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing JAK2 are readily searchable from our website. Different antibodies against the same target such as JAK2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Cardiovascular Diseases, Inflammation, Leukemia, Leukemia, Myeloid, Acute, Neoplasms, Polycythemia, Polycythemia Vera, Primary Myelofibrosis, Thrombocythemia, Essential, Thrombocytosis are some of the diseases may be linked to JAK2.