|The IRF7 irf7 (Catalog #MBS200077) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IRF7 antibody (clone 3D9) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s IRF7 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB) (Cell lysate).
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000~ 2,000. Recommended starting dilution is 1:1,000. Researchers should empirically determine the suitability of the IRF7 irf7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IRF7 irf7 product has the following accession number(s) (GI #4758610) (NCBI Accession #NP_001563). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the IRF7 antibody (clone 3D9) with the following immunoassay(s):
Western Blot (WB) (Cell lysates of Jurkat (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IRF-7 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Interferon regulatory factor (IRF) 7 is a member of the interferon regulatory transcription factor family. IRF7 plays an essential role in virus-activated transcription of type I interferon alpha genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established.
Antigen Species: Human
Clone: Anti-human IRF-7 mAb, clone 3D9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IRF-7 protein.
Immunogen: Recombinant human IRF-7 (1-150aa) purified from E Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing IRF7 are readily searchable from our website. Different antibodies against the same target such as IRF7 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. IRF7 also interacts with the following gene(s): IFIT2, IFIT3, IRAK1, ISG15, MX1, MYD88, OAS2, TBK1, TRAF6.