|The INCENP incenp (Catalog #MBS610766) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The INCENP (Incenp, Inner Centromere Protein) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s INCENP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF).
Suitable for use in Immunofluorescence, Flow Cytometry and Western Blot.
Dilution: Immunofluorescence: 1:400
Flow Cytometry: 1:50
Western Blot: 1:1000. Researchers should empirically determine the suitability of the INCENP incenp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The INCENP incenp product has the following accession number(s) (GI #102467242) (NCBI Accession #NP_064623.2) (Uniprot Accession #Q9NQS7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
INCENP (inner centromere protein antigens 135kD, 155kD) is a chromosomal passenger protein crucial for multiple events that mediate chromosome separation during mitosis (1). At prophase INCENP is associated with chromatin whereas during prometaphase and metaphase it translocates to the inner centromere (1). Depletion of INCENP results in aberrant chromosome alignment at the metaphase plate, incomplete chromosome separation, and disruption of proper spindle formation and cytokinesis (2). INCENP is part of the chromosomal passenger complex that also contains Aurora B, borealin and survivin (2). Aurora B and INCENP are mutually dependent on each other for proper localization (3), and in Drosophila cells and C.elegans embryos that lack INCENP or survivin, Aurora B cannot organize the kinetochores and the midbody (4,5). Phosphorylation on INCENP by CDK1 on Thr59 and Thr388 leads to the association of INCENP with Plk1, another important regulator of mitotic entry and exit (6). Interaction of INCENP with Plk1 is necessary for recruitment of Plk1 to the kinetochores, and the metaphase to anaphase transition (6). Interactions have also been reported between INCENP and heterochromatin protein 1 (HP1) (7) and -tubulin (8).
Immunogen: Synthetic peptide (KLH-coupled) corresponding to amino acids surrounding Pro240 of human INCENP. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing INCENP are readily searchable from our website. Different antibodies against the same target such as INCENP may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. INCENP also interacts with the following gene(s): AURKB, BIRC5, CDCA8.