anti-Il6 antibody product blog
Tags: Antibody; IL-6; Monoclonal Antibody; IL6; anti-IL6 antibody;
The Il6 il6 (Catalog #MBS670076) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse IL-6-UNLB reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s IL-6 can be used in a range of immunoassay formats including, but not limited to, ELISA; Western Blot.Research Application(s): ELISA Detection: MP5-32C11 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying mouse IL-6 protein levels.1,2,4 Purified MP5-20F3 antibody should be paired with biotinylated as the capture antibody, with purified recombinant mouse IL-6 as the standard.
Western Blotting: The purified MP5-32C11 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse IL-6 under reducing conditions.
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications. Researchers should empirically determine the suitability of the Il6 il6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Il6 il6 product has the following accession number(s) (GI #7549769) (NCBI Accession #NP_036721.1) (Uniprot Accession #P20607). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Immunogen: COS-7-expressed recombinant mouse IL-6. Characterization: To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Il6 are readily searchable from our website. Different antibodies against the same target such as Il6 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Atherosclerosis, Cardiovascular Diseases, Inflammation, Insulin Resistance, Kidney Diseases, Leukemia, Liver Diseases, Lung Diseases, Neoplasms, Obesity are some of the diseases may be linked to Rat Anti-Mouse IL-6-UNLB. Uncharacterized Tissue tissues are correlated with this protein.