anti-IL23R antibody product blog
Tags: Antibody; Monoclonal Antibody; IL23R; anti-IL23R antibody; Interleukin 23R;
The IL23R il23r (Catalog #MBS603387) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Interleukin 23R (Interleukin 23 Receptor, IL-23R) (PE) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Interleukin 23R can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: Neat. 10ul labels 1x10e5 cells.
Flow Cytometry Protocol: 1. Cells should be Fc-blocked by treatment with 1ug of human IgG/10e5 cells for 15 minutes at RT prior to staining. Do not wash excess blocking IgG from this reaction.
2. Transfer 25ul of the Fc-blocked cells (1x10e5 cells) or 50ul of packed whole blood to a 5ml tube.
3. Add 10ul of I8443-49A.
4. Incubate for 30-45 minutes at 4 degree C.
5. Following this incubation, remove unreacted I8443-49A reagent by washing the cells twice in 4ml of the same PBS buffer (Note: Whole blood will require an RBC lysis step at this point using any commercially available lysing reagent).
6. Finally, resuspend the cells in 200-400ul of PBS buffer for final flow cytometric analysis.
7. As a control for analysis, cells in a separate tube should be treated with PE-labeled mouse IgG2b antibody. Researchers should empirically determine the suitability of the IL23R il23r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IL23R il23r product has the following accession number(s) (GI #24430212) (NCBI Accession #NP_653302.2) (Uniprot Accession #Q5VWK5). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Interleukin 23R (Interleukin 23 Receptor, IL-23R) (PE) with the following immunoassay(s):
Testing Data (Human peripheral blood mononuclear cells (PBMCs) treated with 50ng/ml PMA, 200ng/ml Calcium Ionomycin, 200ng/ml LPS, 20ng/ml recombinant human IL23 and 40ng/ml recombinant human IL6 overnight to induce Th17 development, were stained with mouse anti-human CD4, APC-conjugated monoclonal antibody and either (A) MBS603387, or with (B) mouse IgG2b, PE isotype control.)
Testing Data
IL-23R is an IL-23-specific binding protein that associates with IL-12 Rb1 to form a functional heterodimeric receptor complex. This complex mediates biological activities that are similar to, but distinct from, those induced by IL-12. IL-23R is expressed by Th1 and NK cells.
Immunogen: Recombinant protein corresponding to aa24-354 of human Interleukin 23 receptor extracellular domain, expressed in NS0 cells (Q5VWK5).