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anti-IL17A antibody product blog

Posted on 2014-01-29 21:05:57 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; IL-17; anti-IL17A antibody; IL17A;
The IL17A il17a (Catalog #MBS220191) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s IL-17 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB).
ELISA: This biotinylated human IL-17 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human IL-17 antibody as the capture reagent and recombinant human IL-17 as the standard.
Western Blot: This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human IL-17 as the positive control.
ELISA: Minimum Dilution: 0.25; Maximum Dilution: 1.0ug/ml
Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml. Researchers should empirically determine the suitability of the IL17A il17a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The IL17A il17a product has the following accession number(s) (GI #4504651) (NCBI Accession #NP_002181.1) (Uniprot Accession #Q16552). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RABBIT ANTI HUMAN INTERLEUKIN-17:Biotin with the following immunoassay(s):
Testing Data #1 (Staining of formalin fixed,paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human interleukin-17 (MBS221587))
Testing Data #1 IL17A.

Testing Data #2 (Formalin fixed, paraffin embedded human breast invasive ductal carcinoma stained with Goat anti Human IL-17A (MBS221587) following heat mediated antigen retrieval with sodium citrate buffer (pH6.0))
Testing Data #2 IL17A.

Rabbit anti Human Interleukin-17 antibody recognizes human IL-17 (Interleukin-17/17A), a 32kDa disulfide-linked homodimer consisting of two 136 amino acid chains, which belongs to the IL-17 family (classified as IL-17A to IL-17F), and signals through the IL-17 receptor (IL-17R/CDw217). IL-17 is produced primarily by activated CD4+ T cells and induces a variety of target cells to secrete inflammatory cytokines, including IL-1beta, IL-6, TNFalpha, IFNgamma and granulocyte colony-stimulating factor, as well as synergizing with other inflammatory cytokines and agonists. The role of IL-17 in bone metabolism has become a focus area for studies into Rheumatoid Arthritis, in which levels of IL-17 are significantly increased and may contribute to bone destruction.

Reconstitution: Reconstitute with 0.5 ml sterile PBS containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. Buffer Solution: Phosphate buffered saline
Antiserum Preparation: Antisera to human IL-17 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Target Species: Human.
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